Free radical scavengers from Cymbopogon citratus (DC.) stapf plants cultivated in bioreactors by the temporary immersion (TIS) principle

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datacite.alternateIdentifier.issn0939-5075
datacite.creatorTapia, A.
datacite.creatorCheel, J.
datacite.creatorTheoduloz, C.
datacite.creatorRodriguez, J.
datacite.creatorSchmeda-Hirschmann, G.
datacite.creatorGerth, A.
datacite.creatorWilken, D.
datacite.creatorJordan, M.
datacite.creatorJimenez-Gonzalez, E.
datacite.creatorGomez-Kosky, R.
datacite.creatorMendoza, E.Q.
datacite.date.issued2007
datacite.subjectCymbopogon citratus, TIS Culture, Free Radical Scavengersen
datacite.titleFree radical scavengers from Cymbopogon citratus (DC.) stapf plants cultivated in bioreactors by the temporary immersion (TIS) principleen
dc.date.accessioned2008-05-06T21:37:03Z
dc.date.available2008-05-06T21:37:03Z
dc.descriptionGuillermo Schmeda-Hirschmann,Alejandro Tapia, Jose Cheel, Instituto de Quımica de Recursos Naturales, Universidad de Talca, Casilla 747, Talca, Chile. E-mail: schmeda@utalca.cl Cristina Theodulozb, Jaime Rodrıguez, Facultad de Ciencias de la Salud, Departamento de Ciencias Basicas Biomedicas, Universidad de Talca, Chileen
dc.description.abstractThe biomass production of Cymbopogon citratus shoots cultivated in bioreactors according to the temporary immersion (TIS) principle was assessed under different growth conditions. The effect of gassing with CO2-enriched air, reduced immersion frequency, vessel size and culture time on total phenolic and flavonoid content and free radical scavenging effect of the methanolic extracts was measured. From the TIS-culture of C. citratus, seven compounds were isolated and identified as caffeic acid (1), chlorogenic acid (2), neochlorogenic acid (3), p-hydroxybenzoic acid (4), p-hydroxybenzoic acid 3-O--d-glucoside (5), glutamic acid (6) and luteolin 6-C-fucopyranoside (7). The occurrence of compounds 1Ð7 and their variability in C. citratus grown under different TIS conditions was determined by HPLC. The free radical scavenging effect of the methanolic extract and compounds was measured by the discoloration of the free radical 1,1- diphenyl-2-picrylhydrazyl (DPPH). The main metabolites in 6- and 8-week-old cultures, both in 5 and 10 l vessels, were chlorogenic acid (2) (100Ð113 mg%) and neochlorogenic acid (3) (80Ð 119 mg%), while in the cultures with CO2-enriched air and reduced immersion frequency the main compound detected in the extracts was glutamic acid (6) (400 and 670 mg% for the green and white biomass and 619 and 630 mg% for the green and white biomass, respectively). The most active compounds, as free radical scavengers, in the DPPH discoloration assay were caffeic acid (1), chlorogenic acid (2), neochlorogenic acid (3) and the flavonoid luteolin 6-C-fucopyranoside (7).en
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dc.identifier.citationZeitschrift Fur Naturforschung C-A Journal of Biosciences 62 (5-6):en
dc.identifier.urihttps://repositorio.utalca.cl/repositorio/handle/1950/4898
dc.languageen
dc.publisherVerlag Z Naturforschen
oaire.resourceTypeArtículoen
utalca.indexArtículo de publicación ISI
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