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Item In vitro propagation of cedar (Cedrela odorata L.) from juvenile shootsAutores: Garcia-Gonzales, R.; Delgado, M.; Gonzalez, Y.; Gonzalez, A.; Garriga, M.; Caligari, P.D.S.; Carrasco, B.; Quiroz, K.Cedrela odorata L. is one of the most important timber species currently traded in the Caribbean and Central America; however, it has been intensively exploited. In vitro techniques and clonal propagation can help to develop new plantations and assist in establishing improvement programs for this species. The aim of this study was to develop a protocol to establish in vitro conditions and to micropropagate this species from nodal explants from juvenile cuttings taken from field trees. Disinfection of node explants with 5% propiconazole CE 25 during 3 min resulted in 100% explant disinfection and 60% morphogenic response on those established explants. Shoot development was optimized by cultivating in vitro node explants in Murashige and Skoog basal medium supplemented with 2 mg L(-1) 6-bencilaminopurine and 3 mg L(-1) naphthaleneacetic acid. This medium resulted in 100% shoot development from the in vitro node explants with a 3.93 cm mean height. Rooting was also stimulated 6 wk after individualization of the regenerated plants on the same micropropagation medium with a mean of 3.9 roots per plant. In vitro plants did not show morphologic differences when compared to ex vitro seeds.Item Biological Fixation of N2 in Mono and Polyspecific Legume Pasture in the Humid Mediterranean Zone of ChileAutores: Espinoza, S.; Ovalle, C.; del Pozo, A.; Zagal, E.; Urquiaga, S.Despite annual legume pasture are of great importance for dryland agricultural systems in Mediterranean environments, there are few studies of N-2 biological fixation (NBF) reported in Chile. In this study the NBF of four annual legume species: subterranean clover (Trifolium subterraneum L.), yellow serradella (Ornithopus compressus L.), arrow-leaf clover (T. vesiculosum L.), and crimson clover (T. incarnatum L.) (Experiment 1), as well as seven mixtures of these species (Experiment 2) were assessed. The NBF was measured by the N-15 natural abundance technique. The objective was to determine NBF in the legume species and in distinct mixtures used. The study was carried out in an Andisol of the Andean Precordillera located in the humid Mediterranean zone of Chile. Pasture was evaluated for biomass; and total N and natural abundance of N-15 were analyzed in plant material samples. In Experiment 1 (monospecific legume species pasture), N derived from fixation ranged between 43 and 147 kg N ha(-1) and where T. vesiculosum and T. subterraneum presented statistical differences (P <= 0.05) in connection with the other species. In the legume mixtures (Experiment 2), N derived by fixation varied between 97 and 214 kg N ha(-1) where the 50-50 mixtures (T. subterraneum and O. compressus, or T. subterraneum and T. vesiculosum, respectively) had the highest N fixation. Fixed N ranged between 12 and 25 kg N t(-1) DM, showing significant differences among mono and polyspecific legume species.
